A spatio-temporal analysis of groundwater level changes in relation to urban growth and groundwater recharge potential for Waukesha County, Wisconsin

The main objective of this study was to spatially and temporally analyze groundwater level changes using geographic information systems and spatial analysis with respect to urban development, groundwater water withdrawal, and groundwater recharge potential. The study focused on Waukesha County in southeastern Wisconsin, where urban development has been accelerating while groundwater has been declining during the last several decades. We analyzed data about groundwater withdrawal, groundwater level, land use/land cover, and precipitation utilizing correlation analysis, Geographically Weighted Regression (GWR), land-use change analysis, and map overlay. As a result, we found that (1) Urban areas increased faster in areas with high recharge potential than in areas with low or moderate recharge potential, (2) The effect of urban growth on groundwater level is highly variable by time and space, and (3) The changes in groundwater level are strongly related to the spatial distribution of groundwater withdrawal. The study pinpoints the need to consider the spatial unevenness of groundwater withdrawal in understanding the changes in groundwater level and groundwater recharge potential for better managing groundwater resources

The Influence of Preoperative Bladder Outlet Obstruction on Continence and Satisfaction in Patients with Stress Urinary Incontinence after Midurethral Sling

Purpose We studied the influence of preoperative bladder outlet obstruction (BOO) on postoperative continence rates and patient satisfaction after the midurethral sling procedure. Methods A total of 159 women who underwent the midurethral sling procedure were evaluated. Using the Blaivas-Groutz nomogram, we assigned the patients were assigned to Group I (n=37, no obstruction), Group II (n=89, mild obstruction), or Group III (n=33, moderate to severe obstruction). Continence rates, patient satisfaction, urinary sensation scale and uroflowmetry were evaluated postoperatively. Results There were no significant differences in continence rates, satisfaction, or postoperative maximal flow rate between the 3 groups. Postoperative urgency was improved after surgery in Groups I and II (P<0.05) but not in Group III. Conclusions BOO does not seem to be a risk factor for failure after the midurethral sling procedure. However, BOO may be considered as a potential factor for persistent storage symptoms after the midurethral sling

Role of Staphylococcal Superantigen in Atopic Dermatitis: Influence on Keratinocytes

Staphylococcus aureus may perform an crucial function in atopic dermatitis (AD), via the secretion of superantigens, including staphylococcal enterotoxins (SE) A or B, and toxic shock syndrome toxin-1 (TSST-1). Dysregulated cytokine production by keratinocytes (KCs) upon exposure to staphylococcal superantigens (SsAgs) may be principally involved in the pathophysiology of AD. We hypothesized that lesional KCs from AD may react differently to SsAgs compared to nonlesional skin or normal skin from nonatopics. We conducted a comparison of HLA-DR or CD1a expression in lesional skin as opposed to that in nonlesional or normal skin by immunohistochemistry (IHC). We also compared, using ELISA, the levels of IL-1α, IL-1β, and TNF-α secreted by cultured KCs from lesional, nonlesional, and normal skin, after the addition of SEA, SEB and TSST-1. IHC revealed that both HLA-DR and CD1a expression increased significantly in the epidermis of lesional skin versus nonlesional or normal skin in quite a similar manner. IL-1α, IL-1β, and TNF-α secretion was also significantly elevated in the cultured KCs from lesional skin after the addition of SsAgs. Our results indicated that KCs from lesional skin appear to react differently to SsAgs and increased proinflammatory cytokine production in response to SsAgs may contribute to the pathogenesis of AD

On the root cause of the host `mass-step' in the Hubble residuals of type Ia supernovae

It is well established that the Hubble residuals of type Ia supernovae (SNe Ia) show the luminosity step with respect to their host galaxy stellar masses. This `mass-step' is taken as an additional correction factor for the SN Ia luminosity standardization. Here we investigate the root cause of the mass-step and propose that the bimodal nature of the host $age$ distribution is responsible for the step. In particular, by using the empirical $nonlinear$ mass-to-age relation of local galaxies, we convert the mass function of SN Ia hosts to their age distribution. We find that the age distribution shows clear bimodality: a younger ($<$ 6 Gyr) group with lower mass ($\sim 10^{9.5}{\rm M}_{\rm sun}$) and an older ($>$ 6 Gyr) group with higher mass ($\sim 10^{10.5}{\rm M}_{\rm sun}$). On the Hubble residual versus host mass plane, the two groups create the mass-step at $\sim 10^{10}{\rm M}_{\rm sun}$. This leads us to conclude that the host galaxy mass-step can be attributed to the bimodal age distribution in relation to a nonlinear relation between galaxy mass and age. We suggest that the mass-step is another manifestation of the old `red sequence' and the young `blue cloud' observed in the galactic color--magnitude diagram.Comment: Accepted for publication in ApJ, 10 pages, 5 figures, 1 tabl

Transvaginal Endoscopic Appendectomy

Since Kalloo and colleagues first reported the feasibility and safety of a peroral transgastric approach in the porcine model in 2004, various groups have reported more complex natural orifice transluminal endoscopic surgery (NOTES) procedures, such as the cholecystectomy, splenectomy and liver biopsy, in the porcine model. Natural orifice access to the abdominal cavity, such as transgastric, transvesical, transcolonic, and transvaginal, has been described. Although a novel, minimally invasive approach to the abdominal cavity is a peroral endoscopic transgastric approach, there are still some challenging issues, such as the risk of infection and leakage, and the method of gastric closure. Hybrid-NOTES is an ideal first step in humans. Human hybrid transvaginal access has been used for years by many surgeons for diagnostic and therapeutic purposes. Here, we report a transvaginal flexible endoscopic appendectomy, with a 5-mm umbilical port using ultrasonic scissors in a 74-year-old woman with acute appendicitis

An Electrophilic Deguelin Analogue Inhibits STAT3 Signaling in H-Ras-Transformed Human Mammary Epithelial Cells: The Cysteine 259 Residue as a Potential Target

Signal transducer and activator of transcription 3 (STAT3) is a point of convergence for numerous oncogenic signals that are often constitutively activated in many cancerous or transformed cells and some stromal cells in the tumor microenvironment. Persistent STAT3 activation in malignant cells stimulates proliferation, survival, angiogenesis, invasion, and tumor-promoting inflammation. STAT3 undergoes activation through phosphorylation on tyrosine 705, which facilitates its dimerization. Dimeric STAT3 translocates to the nucleus, where it regulates the transcription of genes involved in cell proliferation, survival, etc. In the present study, a synthetic deguelin analogue SH48, discovered by virtual screening, inhibited the phosphorylation, nuclear translocation, and transcriptional activity of STAT3 in H-ras transformed human mammary epithelial MCF-10A cells (MCF10A-ras). We speculated that SH48 bearing an alpha,beta-unsaturated carbonyl group could interact with a thiol residue of STAT3, thereby inactivating this transcription factor. Non-electrophilic analogues of SH48 failed to inhibit STAT3 activation, lending support to the above supposition. By utilizing a biotinylated SH48, we were able to demonstrate the complex formation between SH48 and STAT3. SH48 treatment to MCF10A-ras cells induced autophagy, which was verified by staining with a fluorescent acidotropic probe, LysoTracker Red, as well as upregulating the expression of LC3II and p62. In conclusion, the electrophilic analogue of deguelin interacts with STAT3 and inhibits its activation in MCF10A-ras cells, which may account for its induction of autophagic death.